Gene name |
SPBC2G2.11 |
Gene ID |
25/D04 |
Gene synonyms/obsolete |
|
Gene product |
glycylpeptide
N-tetradecanoyltransferase; involved in N-terminal protein
myristoylation |
Entry clone |
Cloned |
ORF length (unspliced) |
1577 |
ORF length (spliced) |
1401 |
Entry clone length |
1577 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
462A:G / 878G:A /
1064T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC2G2.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACAACGAAAATAATAA |
Rev primer name |
SPBC2G2.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAATCATTACAAAACCCATT |
Amino acid length |
466 |
Molecular weight |
53.6 |
Isoelectric point (calc.) |
6.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |