Gene name |
SPBC36.09 |
Gene ID |
25/E08 |
Gene synonyms/obsolete |
sap61 |
Gene product |
zinc finger protein;
zf-C2H2 type; RNA-binding protein; complexed with Cdc5p |
Entry clone |
Cloned |
ORF length (unspliced) |
1586 |
ORF length (spliced) |
1479 |
Entry clone length |
1586 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC36.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAGAAAGCGTGCTCGA |
Rev primer name |
SPBC36.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAATAAACCTTGGGCCTTT |
Amino acid length |
492 |
Molecular weight |
57.2 |
Isoelectric point (calc.) |
5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |