| Gene name |
SPBC1604.08c |
| Gene ID |
25/H09 |
| Gene synonyms/obsolete |
imp1 |
| Gene product |
importin alpha
subunit; armadillo repeat protein; similar to Sp cut15
(paralog); overexpression results in cell cycle defects |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1620 |
| ORF length (spliced) |
|
| Entry clone length |
1620 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1604.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAATCGAGATATTTATC |
| Rev primer name |
SPBC1604.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACATAGCCATATCTTGAGAA |
| Amino acid length |
539 |
| Molecular weight |
59.8 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nuclear envelope;
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |