Gene name |
SPCC4B3.13 |
Gene ID |
25/H10 |
Gene synonyms/obsolete |
|
Gene product |
MatE family
transporter; unknown specificity; similar to Sp SPAC323.07c
and SPAC11D3.06 |
Entry clone |
Cloned |
ORF length (unspliced) |
1620 |
ORF length (spliced) |
|
Entry clone length |
1620 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
549C:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC4B3.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAGGGGAAAATGATCC |
Rev primer name |
SPCC4B3.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCCTCATCAAACTGACGA |
Amino acid length |
539 |
Molecular weight |
58.8 |
Isoelectric point (calc.) |
8 |
Signal SEQ |
|
No. of transmembrane domain |
11 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQRILAILLLI/LGAPVAVALTL/LDWGLYGLWI |
Localization (YFP) |
vacuole membrane |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |