Gene name |
SPBC3F6.06c |
Gene ID |
26/A04 |
Gene synonyms/obsolete |
SPBC342.01c |
Gene product |
glycosyl transferase
family ALG8 |
Entry clone |
Cloned |
ORF length (unspliced) |
1627 |
ORF length (spliced) |
1521 |
Entry clone length |
1627 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
509T:C / 1394T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC3F6.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTATCTGAGTTTGAGAA |
Rev primer name |
SPBC3F6.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGCTTCTTTTTTGTTACGC |
Amino acid length |
506 |
Molecular weight |
59.2 |
Isoelectric point (calc.) |
9.7 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
12 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LWTLGRLLHI |
Localization (YFP) |
Golgi; vacuole
membrane |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |