| Gene name |
SPBC14C8.17c |
| Gene ID |
26/A05 |
| Gene synonyms/obsolete |
|
| Gene product |
WD repeat protein;
involved in transcriptional activation; SAGA complex
(Spt-Ada-Gcn5-Acetyltransferase); ADA complex |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1628 |
| ORF length (spliced) |
1581 |
| Entry clone length |
1628 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
8C:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC14C8.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAGCAGAAGAGAGTGA |
| Rev primer name |
SPBC14C8.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGAAGTCATTATTTGGTAT |
| Amino acid length |
526 |
| Molecular weight |
58.4 |
| Isoelectric point (calc.) |
4.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
| Microscope used for
observation |
DeltaVision |