| Gene name |
SPCC584.13 |
| Gene ID |
26/B04 |
| Gene synonyms/obsolete |
|
| Gene product |
amino acid permease
family; similar to Sp SPBC15C4.04C and SPAPB24D3.02C and
SPCC74.04 and SPAC1039.01 and SPAC11D3.08C and SPAC9.10 and
SPCC794.03 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1635 |
| ORF length (spliced) |
|
| Entry clone length |
1635 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
575T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC584.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTATTTTCAAAAAATC |
| Rev primer name |
SPCC584.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTTTTCTTCAAAGTCGAA |
| Amino acid length |
544 |
| Molecular weight |
59.5 |
| Isoelectric point (calc.) |
7.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
12 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytoplasmic dots;
vacuole? |
| Comments for localization |
bright dots by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |