| Gene name |
SPAC22H10.11c |
| Gene ID |
26/E02 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; fungal conserved protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1890 |
| ORF length (spliced) |
|
| Entry clone length |
1890 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1095T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC22H10.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGCTAACGAATGGGCG |
| Rev primer name |
SPAC22H10.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATAGCCAGAAGATTCTAAG |
| Amino acid length |
629 |
| Molecular weight |
71.2 |
| Isoelectric point (calc.) |
4.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLSAVPTPLEL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus) |
| Microscope used for
observation |
DeltaVision |