Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23H3.11c
Gene ID 26/E03
Gene synonyms/obsolete
Gene product glucosidase activity; involved in beta-1,6 glucan biosynthesis; involved in cell wall organization and biogenesis
Entry clone Cloned
ORF length (unspliced) 1890
ORF length (spliced)
Entry clone length 1890
No. of intron 0
Sequence status Finished
Sequence results 678A:G / 1001C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23H3.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAAAAGGTCATAGCGA
Rev primer name SPAC23H3.11.Rv
Rev primer SEQ AGAAAGCTGGGTAAGTGTTACATTTATGCATT
Amino acid length 629
Molecular weight 70.7
Isoelectric point (calc.) 4.6
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFFLFVLSLGI
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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