Gene name |
SPAC23H3.11c |
Gene ID |
26/E03 |
Gene synonyms/obsolete |
|
Gene product |
glucosidase activity;
involved in beta-1,6 glucan biosynthesis; involved in cell
wall organization and biogenesis |
Entry clone |
Cloned |
ORF length (unspliced) |
1890 |
ORF length (spliced) |
|
Entry clone length |
1890 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
678A:G / 1001C:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC23H3.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAAAAGGTCATAGCGA |
Rev primer name |
SPAC23H3.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTGTTACATTTATGCATT |
Amino acid length |
629 |
Molecular weight |
70.7 |
Isoelectric point (calc.) |
4.6 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFFLFVLSLGI |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |