| Gene name |
SPAC16.04 |
| Gene ID |
26/F01 |
| Gene synonyms/obsolete |
|
| Gene product |
dihydrouridine
synthase; involved in tRNA modification |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1902 |
| ORF length (spliced) |
1854 |
| Entry clone length |
1902 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
86T:C / 1719A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC16.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGAATTGGAGACTGG |
| Rev primer name |
SPAC16.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCCCTCTGCCTCTTCCACA |
| Amino acid length |
617 |
| Molecular weight |
69.6 |
| Isoelectric point (calc.) |
7.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
22 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus; one nuclear
dot |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |