Gene name |
SPBC25B2.03 |
Gene ID |
26/H09 |
Gene synonyms/obsolete |
|
Gene product |
zinc finger protein;
zf-C2H2 type |
Entry clone |
Cloned |
ORF length (unspliced) |
1944 |
ORF length (spliced) |
1665 |
Entry clone length |
1944 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
448C:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC25B2.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAAATACAATTGGGAA |
Rev primer name |
SPBC25B2.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATGACCTCTTTCGAGTTCA |
Amino acid length |
554 |
Molecular weight |
61.8 |
Isoelectric point (calc.) |
5.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LANVEQLML |
Localization (YFP) |
periphery; ER?;
Golgi?; vacuole membrane |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |