Gene name |
SPCC1739.13 |
Gene ID |
26/H10 |
Gene synonyms/obsolete |
hsp70 |
Gene product |
heat shock protein 70
family; similar to Sp SPAC13G7.02 (paralog) |
Entry clone |
Cloned |
ORF length (unspliced) |
1944 |
ORF length (spliced) |
|
Entry clone length |
1944 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
892G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1739.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCAAATCAATCGGAAT |
Rev primer name |
SPCC1739.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCAACCTCCTCAACCTCA |
Amino acid length |
647 |
Molecular weight |
70.2 |
Isoelectric point (calc.) |
4.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIAGLNVLRI |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |