Gene name |
SPAC4H3.03c |
Gene ID |
26/H11 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; conserved protein (mainly bacterial); no
apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1950 |
ORF length (spliced) |
|
Entry clone length |
1950 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
49A:G / 138A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC4H3.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTCCTAACGCCAGAGT |
Rev primer name |
SPAC4H3.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAATTTCATTGAAAATAGAA |
Amino acid length |
649 |
Molecular weight |
74.4 |
Isoelectric point (calc.) |
5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LWVALDRALRI |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |