| Gene name |
SPCC1223.03c |
| Gene ID |
26/H12 |
| Gene synonyms/obsolete |
gut2 |
| Gene product |
glycerol-3-phosphate
dehydrogenase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1950 |
| ORF length (spliced) |
|
| Entry clone length |
1950 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
717A:G / 1610C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1223.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTTCAATCTTCAAACG |
| Rev primer name |
SPCC1223.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTGACTTCGAACTGTTTA |
| Amino acid length |
649 |
| Molecular weight |
71.8 |
| Isoelectric point (calc.) |
7.3 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |