Gene name |
SPCC338.17c |
Gene ID |
27/B06 |
Gene synonyms/obsolete |
rad21 |
Gene product |
mitotic cohesin;
involved in chromosome cohesion, DNA repair |
Entry clone |
Cloned |
ORF length (unspliced) |
1967 |
ORF length (spliced) |
1887 |
Entry clone length |
1967 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC338.17.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTCTATTCAGAGGCCAT |
Rev primer name |
SPCC338.17.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAGTGATGAAAGTAGCATT |
Amino acid length |
628 |
Molecular weight |
67.8 |
Isoelectric point (calc.) |
4.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLAPVDDLNL |
Localization (YFP) |
nucleus;
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|