| Gene name |
SPCC4B3.10c |
| Gene ID |
27/C04 |
| Gene synonyms/obsolete |
ipk1 |
| Gene product |
inositol
1,3,4,5,6-pentakisphosphate (IP5) kinase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1979 |
| ORF length (spliced) |
1923 |
| Entry clone length |
1979 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
186A:C / 411A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC4B3.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTCTAAAAAGTATGTC |
| Rev primer name |
SPCC4B3.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTTATGCTGCTCCGGCAT |
| Amino acid length |
640 |
| Molecular weight |
72.7 |
| Isoelectric point (calc.) |
9.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
132 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDDLQKHLTL/LMKHFYCLQL |
| Localization (YFP) |
cytosol |
| Comments for localization |
except nucleolus |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|