Gene name |
SPBC3E7.09 |
Gene ID |
27/C05 |
Gene synonyms/obsolete |
|
Gene product |
conserved hypothetical
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1980 |
ORF length (spliced) |
|
Entry clone length |
1980 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC3E7.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTCAAAAGAAGATTGTC |
Rev primer name |
SPBC3E7.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGCATACTGAATGGCTCCTA |
Amino acid length |
659 |
Molecular weight |
74 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGTFTALNL/LLHELNSTLQL/LFALQAKLEL |
Localization (YFP) |
periphery |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |