Gene name |
SPBC1683.13c |
Gene ID |
27/C10 |
Gene synonyms/obsolete |
|
Gene product |
transcriptional
regulator; zinc finger protein; similar to Sp SPCC757.04 and
SPCC777.02 |
Entry clone |
Cloned |
ORF length (unspliced) |
1991 |
ORF length (spliced) |
1857 |
Entry clone length |
1991 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1683.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAAATGAAACCCCGACA |
Rev primer name |
SPBC1683.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATATTTTACATTGGGAGAA |
Amino acid length |
618 |
Molecular weight |
72.2 |
Isoelectric point (calc.) |
6.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQTLLVLGI |
Localization (YFP) |
nucleus |
Comments for localization |
nuclear dots by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal,
DeltaVision |