Gene name |
SPAC6F12.14 |
Gene ID |
27/E10 |
Gene synonyms/obsolete |
cut23; apc8 |
Gene product |
anaphase-promoting
complex (APC); cyclosome; TPR repeat protein |
Entry clone |
Cloned |
ORF length (unspliced) |
2028 |
ORF length (spliced) |
1698 |
Entry clone length |
2028 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
552A:G / 1960T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC6F12.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTGTGTCGCTGAATAC |
Rev primer name |
SPAC6F12.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCATATGAATGCTCCATC |
Amino acid length |
565 |
Molecular weight |
65.8 |
Isoelectric point (calc.) |
5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSEVLNGDLEL |
Localization (YFP) |
SPB?; nuclear
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |