Gene name |
SPBC365.12c |
Gene ID |
27/F09 |
Gene synonyms/obsolete |
ish1; isp1 |
Gene product |
involved in stress
response; similar to Sp SPAC23C4.05c (C-terminal);
non-essential; overexpression lethal |
Entry clone |
Cloned# |
ORF length (unspliced) |
2055 |
ORF length (spliced) |
|
Entry clone length |
2055 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC365.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGTTCTCGATCAGTGGC |
Rev primer name |
SPBC365.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAAAACCCTTTGGGCTTTC |
Amino acid length |
684 |
Molecular weight |
75.6 |
Isoelectric point (calc.) |
9.7 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |