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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC63.03
Gene ID 27/H05
Gene synonyms/obsolete
Gene product DNAJ domain protein; no Sc ortholog
Entry clone Cloned
ORF length (unspliced) 2097
ORF length (spliced) 1929
Entry clone length 2097
No. of intron 3
Sequence status Finished
Sequence results 231A:G / 278A:G / 980A:C / 1253A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC63.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATGAAGCGGATTCTTG
Rev primer name SPCC63.03.Rv
Rev primer SEQ AGAAAGCTGGGTAGCGAAAATGTAAACAATGA
Amino acid length 642
Molecular weight 72.2
Isoelectric point (calc.) 9.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 489
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Confocal

Image information
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