| Gene name |
SPBC947.10 |
| Gene ID |
27/H06 |
| Gene synonyms/obsolete |
|
| Gene product |
zf-C3HC4 type (RING
finger); ubiquitin ligase (E3) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2099 |
| ORF length (spliced) |
2031 |
| Entry clone length |
2099 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC947.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTATTTGCACCGGCTCC |
| Rev primer name |
SPBC947.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACGGCGGGAAGATGGCAG |
| Amino acid length |
676 |
| Molecular weight |
76.9 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
7 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVRFSSLYI/LGASVLRLYL/LAIFIDSELIL/LWMLFQVLVL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal; DeltaVision
|