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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC926.04c
Gene ID 28/A02
Gene synonyms/obsolete hsp90; swo1
Gene product chaperone; heat shock protein 90 homolog
Entry clone Cloned
ORF length (unspliced) 2115
ORF length (spliced)
Entry clone length 2115
No. of intron 0
Sequence status Finished
Sequence results 165T:C / 1245T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC926.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGAACACAGAAACTTT
Rev primer name SPAC926.04.Rv
Rev primer SEQ AGAAAGCTGGGTAATCGACTTCCTCCATCTTG
Amino acid length 704
Molecular weight 80.5
Isoelectric point (calc.) 4.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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