| Gene name |
SPAC1952.16 |
| Gene ID |
28/D06 |
| Gene synonyms/obsolete |
|
| Gene product |
FCH domain; RhoGAp
(GTPase activator towards Rho/Rac/Cdc42-like small GTPases);
Fes/CIP4 homology domain; similar to Sp SPAC2F7.18C |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2339 |
| ORF length (spliced) |
2055 |
| Entry clone length |
2339 |
| No. of intron |
6 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1952.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTGGGACGGCTTTTCAAA |
| Rev primer name |
SPAC1952.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAATGGTCTAAATCTTCGT |
| Amino acid length |
684 |
| Molecular weight |
78.4 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LHKKLGEQLNI/LASALSQLDI/LSSITCSLRL |
| Localization (YFP) |
cytoplasmic dots;
cytosol |
| Comments for localization |
bright dots by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |