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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23H4.15
Gene ID 28/D10
Gene synonyms/obsolete
Gene product involved in RNA processing; involved in RNA modification
Entry clone Cloned
ORF length (unspliced) 2352
ORF length (spliced)
Entry clone length 2352
No. of intron 0
Sequence status Finished
Sequence results 508G:A / 1611T:C / 1742A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23H4.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTCATCATCATCGATC
Rev primer name SPAC23H4.15.Rv
Rev primer SEQ AGAAAGCTGGGTATGAATCAATATCCGTAACC
Amino acid length 783
Molecular weight 90.1
Isoelectric point (calc.) 5.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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