Gene name |
SPAC3C7.04 |
Gene ID |
28/D11 |
Gene synonyms/obsolete |
|
Gene product |
transcriptional
regulator; zinc finger protein; zf-fungal Zn(2)-Cys(6)
binuclear cluster domain; putative metabolic control; similar
to Sp thi1 |
Entry clone |
Cloned |
ORF length (unspliced) |
2352 |
ORF length (spliced) |
|
Entry clone length |
2352 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1267A:G /
1940A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC3C7.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCCATGAATTTTCACC |
Rev primer name |
SPAC3C7.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGGACAAACGTAATCTAGC |
Amino acid length |
783 |
Molecular weight |
87.9 |
Isoelectric point (calc.) |
5.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPTVVPELEI |
Localization (YFP) |
cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
DeltaVision |