| Gene name |
SPBC3F6.04c |
| Gene ID |
29/C09 |
| Gene synonyms/obsolete |
|
| Gene product |
ribonucleoprotein
(RNP) complex; processome component; involved in rRNA
processing; conserved eukaryotic protein; involved in ribosome
biogenesis and assembly |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2535 |
| ORF length (spliced) |
2484 |
| Entry clone length |
2535 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC3F6.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGTAAAAATGGATCACA |
| Rev primer name |
SPBC3F6.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGACAGCCGGATCAAAATGC |
| Amino acid length |
827 |
| Molecular weight |
95.1 |
| Isoelectric point (calc.) |
6.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAQQFPSALGI/LSDVCKKLYI |
| Localization (YFP) |
nucleolus>nucleus |
| Comments for localization |
nucleolar dots by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |