| Gene name |
SPAC4F8.11 |
| Gene ID |
29/C10 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; WD repeat protein; similar to human peroxisomal
targeting signal receptor; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2541 |
| ORF length (spliced) |
|
| Entry clone length |
2541 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
258A:G / 548T:C /
2225T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC4F8.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTTCACGAAACTCAAA |
| Rev primer name |
SPAC4F8.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATCAGAGCACGTTTATCT |
| Amino acid length |
846 |
| Molecular weight |
93.2 |
| Isoelectric point (calc.) |
7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKKCVYCELPL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
| Microscope used for
observation |
Leica |