Gene name |
SPAC22F8.08 |
Gene ID |
29/D08 |
Gene synonyms/obsolete |
sec24 |
Gene product |
SEC23/SEC24 family;
SEC24 subfamily; COPII-coated vesicle component; involved in
intracellular protein transport; involved in ER to Golgi
transport |
Entry clone |
Cloned |
ORF length (unspliced) |
2781 |
ORF length (spliced) |
|
Entry clone length |
2781 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
21C:T / 712T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC22F8.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTCAGGAGGACGCTTT |
Rev primer name |
SPAC22F8.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTCACGAACTTTCTCTTTA |
Amino acid length |
926 |
Molecular weight |
101.9 |
Isoelectric point (calc.) |
6.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRLLPLLCL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |