Gene name |
SPBC1347.01c |
Gene ID |
29/E01 |
Gene synonyms/obsolete |
SPBC215.16c |
Gene product |
deoxycytidyl
transferase; involved in DNA repair; involved in mutagenic
translesion DNA replication; BRCT domain; impB/mucB/samB
family domain; BRCT domain |
Entry clone |
Cloned# |
ORF length (unspliced) |
2808 |
ORF length (spliced) |
|
Entry clone length |
2808 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC1347.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTTTTAATCAAAGGAA |
Rev primer name |
SPBC1347.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAAAATCATTAATGGAGGT |
Amino acid length |
935 |
Molecular weight |
106.5 |
Isoelectric point (calc.) |
9.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
5 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQRNIPPLMI |
Localization (YFP) |
nucleolus>nucleus;
spindle microtubules |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |