| Gene name |
SPAC13F5.06c |
| Gene ID |
29/E02 |
| Gene synonyms/obsolete |
sec10 |
| Gene product |
involved in secretory
pathway; involved in exocytosis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2810 |
| ORF length (spliced) |
2436 |
| Entry clone length |
2810 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
27T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC13F5.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTACAAAAAACGCGTT |
| Rev primer name |
SPAC13F5.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACTGCTCTTTGGGGGCAAT |
| Amino acid length |
811 |
| Molecular weight |
92.5 |
| Isoelectric point (calc.) |
6.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LREKFAQVLEL/LRSLFRLSL/LRFDLPENLKL |
| Localization (YFP) |
SPB; periphery at site
of septum formation; cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |