| Gene name |
SPAC23H4.01c |
| Gene ID |
29/E11 |
| Gene synonyms/obsolete |
SPAP27G11.01 |
| Gene product |
involved in ergosterol
biosynthesis; oxysterol binding protein; ankyrin repeat
protein; pleckstrin homology domain; similar to Sp
SPBC2F12.05c |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2838 |
| ORF length (spliced) |
|
| Entry clone length |
2838 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
2401T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC23H4.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAACTGTGGAGATACG |
| Rev primer name |
SPAC23H4.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCATAATTTAGGACATGAA |
| Amino acid length |
945 |
| Molecular weight |
106.8 |
| Isoelectric point (calc.) |
6.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
157 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |