| Gene name |
SPBC215.12 |
| Gene ID |
30/A03 |
| Gene synonyms/obsolete |
cwf10; spef2;
snu114 |
| Gene product |
involved in mRNA
splicing; similar to human U5 snRNP GTPase-U5 snRNP-specific
ribosomal translocase EF-2 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3093 |
| ORF length (spliced) |
2952 |
| Entry clone length |
3093 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
3017A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC215.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAGAGGATTTATATGA |
| Rev primer name |
SPBC215.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAAAGAGAGTACGACACCT |
| Amino acid length |
983 |
| Molecular weight |
111.1 |
| Isoelectric point (calc.) |
5.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LHDMPIVLVL/LSSFAKLYI/LYKLHTLTI |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |