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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17H9.02
Gene ID 30/A02
Gene synonyms/obsolete
Gene product DEAD/DEAH box helicase; involved in mRNA export
Entry clone Cloned
ORF length (unspliced) 3093
ORF length (spliced)
Entry clone length 3093
No. of intron 0
Sequence status Finished
Sequence results 510A:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17H9.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAGAAAACAGTACAGA
Rev primer name SPAC17H9.02.Rv
Rev primer SEQ AGAAAGCTGGGTAGAGGTATAGAGAGGCCGAA
Amino acid length 1030
Molecular weight 118.4
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSLSLRKLEI
Localization (YFP) nucleus; one nuclear dot
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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