Gene name |
SPAC17H9.02 |
Gene ID |
30/A02 |
Gene synonyms/obsolete |
|
Gene product |
DEAD/DEAH box
helicase; involved in mRNA export |
Entry clone |
Cloned |
ORF length (unspliced) |
3093 |
ORF length (spliced) |
|
Entry clone length |
3093 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
510A:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC17H9.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAGAAAACAGTACAGA |
Rev primer name |
SPAC17H9.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGAGGTATAGAGAGGCCGAA |
Amino acid length |
1030 |
Molecular weight |
118.4 |
Isoelectric point (calc.) |
6.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSLSLRKLEI |
Localization (YFP) |
nucleus; one nuclear
dot |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |