| Gene name |
SPBC2G2.02 |
| Gene ID |
30/B10 |
| Gene synonyms/obsolete |
|
| Gene product |
phosphatidylinositol
phosphate phosphatase; synaptojanin-like |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3231 |
| ORF length (spliced) |
|
| Entry clone length |
3231 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1352A:G / 1644A:G /
2110T:C / 2423A:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC2G2.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAATGTCTGCTGAGGGA |
| Rev primer name |
SPBC2G2.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACACAAGCGGTTTCCAAGAA |
| Amino acid length |
1076 |
| Molecular weight |
121.8 |
| Isoelectric point (calc.) |
8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDFDITTRLQL |
| Localization (YFP) |
cytoplasmic dots at
cell tip and site of septum formation; cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |