| Gene name |
SPAC31G5.15 |
| Gene ID |
30/B11 |
| Gene synonyms/obsolete |
|
| Gene product |
phosphatidylserine
decarboxylase; C2 domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3233 |
| ORF length (spliced) |
2943 |
| Entry clone length |
3233 |
| No. of intron |
6 |
| Sequence status |
Finished |
| Sequence results |
824T:C / 1776T:C /
1788A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC31G5.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTTTTACGAAAAGCTG |
| Rev primer name |
SPAC31G5.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGAATCTGACTTTGAATGG |
| Amino acid length |
980 |
| Molecular weight |
109.7 |
| Isoelectric point (calc.) |
6.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLGFVFLEI |
| Localization (YFP) |
cytosol; periphery at
site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
| Microscope used for
observation |
Confocal |