| Gene name |
SPBC146.01 |
| Gene ID |
30/C02 |
| Gene synonyms/obsolete |
SPBP35G2.15 |
| Gene product |
RNA polymerase II
holoenzyme component; mediator complex |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3238 |
| ORF length (spliced) |
3192 |
| Entry clone length |
3238 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
1970A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC146.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGAGACAGAGCATTGCC |
| Rev primer name |
SPBC146.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATCACAACTTGCCATATG |
| Amino acid length |
1063 |
| Molecular weight |
118.4 |
| Isoelectric point (calc.) |
10 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nuclear dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |