Gene name |
SPAC19A8.01c |
Gene ID |
30/C04 |
Gene synonyms/obsolete |
sec73; sec7c;
SPAC23H3.01 |
Gene product |
Sec7 domain;
cytohesin-like protein; similar to Sp sec71 and sec72 and
sec74 |
Entry clone |
Cloned |
ORF length (unspliced) |
3249 |
ORF length (spliced) |
|
Entry clone length |
3249 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC19A8.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACCTTCAGAATTCCTTC |
Rev primer name |
SPAC19A8.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTATCAGAAGTAGCAGAA |
Amino acid length |
1082 |
Molecular weight |
122.5 |
Isoelectric point (calc.) |
6.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
periphery at cell tip
and site of septum formation; cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |