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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC28F2.03
Gene ID 31/E03
Gene synonyms/obsolete ppi1; cpy2
Gene product cyclophilin; peptidyl-prolyl cis-trans isomerase
Entry clone Cloned
ORF length (unspliced) 489
ORF length (spliced)
Entry clone length 489
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC28F2.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCCAACTGCTTTTTTGA
Rev primer name SPBC28F2.03.Rv
Rev primer SEQ AGAAAGCTGGGTAGACGGTACCACACTTGTCA
Amino acid length 162
Molecular weight 17.4
Isoelectric point (calc.) 8.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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