Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23G3.04
Gene ID 31/E12
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan; similar to C. albicans CA49C10.10 (possibly spurious)
Entry clone Cloned
ORF length (unspliced) 585
ORF length (spliced)
Entry clone length 585
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23G3.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCGAAACTTTAGTATT
Rev primer name SPAC23G3.04.Rv
Rev primer SEQ AGAAAGCTGGGTAAATACCAGTTGCAGATTCA
Amino acid length 194
Molecular weight 21.1
Isoelectric point (calc.) 10.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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