| Gene name |
SPBC3B9.13c |
| Gene ID |
31/F01 |
| Gene synonyms/obsolete |
rpa1-2; rpaP1-3;
rpa3 |
| Gene product |
60s acidic ribosomal
protein; similar to Sp rpp101 and rpp103 (paralogs) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
589 |
| ORF length (spliced) |
333 |
| Entry clone length |
589 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC3B9.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGCCTCCGAACTTGC |
| Rev primer name |
SPBC3B9.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCAAACAATCCGAATCCC |
| Amino acid length |
110 |
| Molecular weight |
11.1 |
| Isoelectric point (calc.) |
3.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |