Gene name |
SPBC21C3.13 |
Gene ID |
31/F02 |
Gene synonyms/obsolete |
rps1901; rps19-1 |
Gene product |
40S ribosomal protein
(S19.1/S19A subunit) |
Entry clone |
Cloned |
ORF length (unspliced) |
591 |
ORF length (spliced) |
435 |
Entry clone length |
591 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
195A:G / 202T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC21C3.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGGCGTTTCTGTTAA |
Rev primer name |
SPBC21C3.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCGCTTTCTTCTTCCAAA |
Amino acid length |
144 |
Molecular weight |
16.1 |
Isoelectric point (calc.) |
10.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |