Gene name |
SPBC543.08 |
Gene ID |
31/H06 |
Gene synonyms/obsolete |
|
Gene product |
involved in inositol
phospholipid biosynthesis |
Entry clone |
Cloned |
ORF length (unspliced) |
753 |
ORF length (spliced) |
|
Entry clone length |
753 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
268T:C / 269T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC543.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTGAAAAAACTGCTAG |
Rev primer name |
SPBC543.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTAATCGGAGGATCCTAAA |
Amino acid length |
250 |
Molecular weight |
28.7 |
Isoelectric point (calc.) |
8.9 |
Signal SEQ |
|
No. of transmembrane domain |
5 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |