Gene name |
SPBC405.04c |
Gene ID |
31/H05 |
Gene synonyms/obsolete |
ypt7 |
Gene product |
small GTPase; Ras
family; involved in intracellular protein transport and the
regulation of vacuole fusion |
Entry clone |
Cloned |
ORF length (unspliced) |
753 |
ORF length (spliced) |
618 |
Entry clone length |
753 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC405.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCCGGCAAAAAGAAGCA |
Rev primer name |
SPBC405.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACAGTAACATGAAGTTTTT |
Amino acid length |
205 |
Molecular weight |
22.9 |
Isoelectric point (calc.) |
4.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |