| Gene name |
SPBC24C6.06 |
| Gene ID |
32/F08 |
| Gene synonyms/obsolete |
gpa1 |
| Gene product |
guanine
nucleotide-binding protein (alpha-1 subunit); non-essential;
involved in conjugation, sporulation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1224 |
| ORF length (spliced) |
|
| Entry clone length |
1224 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
333A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC24C6.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGATGCATGTCGAGTAA |
| Rev primer name |
SPBC24C6.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAACATAAGTGACTGTAGA |
| Amino acid length |
407 |
| Molecular weight |
46.2 |
| Isoelectric point (calc.) |
7.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
periphery;
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |