| Gene name |
SPBC646.13 |
| Gene ID |
32/F09 |
| Gene synonyms/obsolete |
moc1; sds23;
psp1 |
| Gene product |
CBS domain protein;
involved in destruction box mediated ubiquitinylation;
phosphatase and 20S cyclosome/APC for the onset of
anaphase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1227 |
| ORF length (spliced) |
|
| Entry clone length |
1227 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
566T:C / 602A:G /
839T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC646.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTTTGTCAACTCAATC |
| Rev primer name |
SPBC646.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCGACGTTGTGTATCTACA |
| Amino acid length |
408 |
| Molecular weight |
44.2 |
| Isoelectric point (calc.) |
8.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |