Gene name |
SPAC23G3.10c |
Gene ID |
32/G05 |
Gene synonyms/obsolete |
|
Gene product |
involved in
transcriptional regulation, chromatin remodeling |
Entry clone |
Cloned |
ORF length (unspliced) |
1278 |
ORF length (spliced) |
|
Entry clone length |
1278 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC23G3.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAATAATAGCCGTTT |
Rev primer name |
SPAC23G3.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTTCTTAGCAGAAGCATTT |
Amino acid length |
425 |
Molecular weight |
49.7 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSKAFANILGI |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleus>>cytosol (intranuclear microtubule
bundle?) |
Microscope used for
observation |
Leica |