Gene name |
SPBC16C6.01c |
Gene ID |
33/A04 |
Gene synonyms/obsolete |
SPBC543.11c |
Gene product |
SET domain; histone
lysine methyltransferase; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1422 |
ORF length (spliced) |
|
Entry clone length |
1422 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1056T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC16C6.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACAATTCCAAGCAATT |
Rev primer name |
SPBC16C6.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCTTCAAAATTTTCAGTA |
Amino acid length |
473 |
Molecular weight |
55.5 |
Isoelectric point (calc.) |
6.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQIFYHELNL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
dots by over
expression? |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |