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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC683.01c
Gene ID 33/B12
Gene synonyms/obsolete SPAC25B8.19c
Gene product conserved hypothetical; zinc finger protein; zf-C2H2 type; zinc finger protein; zf-C2H2 type; similar to Sp SPAC3H1.11
Entry clone Cloned
ORF length (unspliced) 1569
ORF length (spliced)
Entry clone length 1569
No. of intron 0
Sequence status Finished
Sequence results 46A:G / 384A:G / 517G:deletion / 518C:deletion / 519A:deletion
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC683.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCTCAGATAACACTCC
Rev primer name SPAC683.01.Rv
Rev primer SEQ AGAAAGCTGGGTACAAACCATGAATGCGTTGA
Amino acid length 522
Molecular weight 55
Isoelectric point (calc.) 7.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots
Comments for localization bright signal
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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