| Gene name |
SPBC530.14c |
| Gene ID |
33/C06 |
| Gene synonyms/obsolete |
dsk1 |
| Gene product |
serine/threonine
protein kinase; cell cycle stage dependent phosphorylation and
localization; mitotic regulator; involved in mRNA
splicing |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1635 |
| ORF length (spliced) |
|
| Entry clone length |
1635 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
866C:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC530.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAAGTGACGGGTCGAG |
| Rev primer name |
SPBC530.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACGAATTTCAGTAGCCCAT |
| Amino acid length |
544 |
| Molecular weight |
61 |
| Isoelectric point (calc.) |
8.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol during
interphase; nucleus; SPB; periphery at site of septum
formation during mitosis |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |